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rat bdnf elisa kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology rat bdnf elisa kit
    Persistent hyperalgesia in the RPP model (A and B) Paw withdrawal latency (A) and 50% paw withdrawal threshold (B) of each rat ( n = 3–6). (C) Representative histological images of DRG from T10-S4 by H&E staining. Arrow, Schwann cell; ∗, nuclei; #, cytoplasm. Scale bars, 50 μm and 25 μm. (D and E) Representative IHC images (D) and quantification (E) of <t>BDNF</t> in the DRG from T10-S4. Brown DAB staining indicates BDNF immunopositivity. The staining intensity was semi-quantitatively scored as follows: (1) weak, (2) moderate, and (3) intense. Scale bars, 50 μm. ( n = 3 biological replicates per group). (F) Serum BDNF level on days 12 and 24 of RPP modeling ( n = 3). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, two-way ANOVA (A and B) or one-way ANOVA (E and F).
    Rat Bdnf Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A pharmacological rat model of recurrent pelvic pain exhibiting hyperalgesia and depression-like behaviors"

    Article Title: A pharmacological rat model of recurrent pelvic pain exhibiting hyperalgesia and depression-like behaviors

    Journal: iScience

    doi: 10.1016/j.isci.2026.115059

    Persistent hyperalgesia in the RPP model (A and B) Paw withdrawal latency (A) and 50% paw withdrawal threshold (B) of each rat ( n = 3–6). (C) Representative histological images of DRG from T10-S4 by H&E staining. Arrow, Schwann cell; ∗, nuclei; #, cytoplasm. Scale bars, 50 μm and 25 μm. (D and E) Representative IHC images (D) and quantification (E) of BDNF in the DRG from T10-S4. Brown DAB staining indicates BDNF immunopositivity. The staining intensity was semi-quantitatively scored as follows: (1) weak, (2) moderate, and (3) intense. Scale bars, 50 μm. ( n = 3 biological replicates per group). (F) Serum BDNF level on days 12 and 24 of RPP modeling ( n = 3). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, two-way ANOVA (A and B) or one-way ANOVA (E and F).
    Figure Legend Snippet: Persistent hyperalgesia in the RPP model (A and B) Paw withdrawal latency (A) and 50% paw withdrawal threshold (B) of each rat ( n = 3–6). (C) Representative histological images of DRG from T10-S4 by H&E staining. Arrow, Schwann cell; ∗, nuclei; #, cytoplasm. Scale bars, 50 μm and 25 μm. (D and E) Representative IHC images (D) and quantification (E) of BDNF in the DRG from T10-S4. Brown DAB staining indicates BDNF immunopositivity. The staining intensity was semi-quantitatively scored as follows: (1) weak, (2) moderate, and (3) intense. Scale bars, 50 μm. ( n = 3 biological replicates per group). (F) Serum BDNF level on days 12 and 24 of RPP modeling ( n = 3). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, two-way ANOVA (A and B) or one-way ANOVA (E and F).

    Techniques Used: Staining

    Comorbid depression-like behavior in the RPP model (A and B) Serum BDNF (A) and 5-HT (B) levels in the RPP model ( n = 6). (C and D) Sucrose preference ratio (C) and immobility time (D) in the RPP model ( n = 8). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, paired t test (A and B) or two-way ANOVA (C and D).
    Figure Legend Snippet: Comorbid depression-like behavior in the RPP model (A and B) Serum BDNF (A) and 5-HT (B) levels in the RPP model ( n = 6). (C and D) Sucrose preference ratio (C) and immobility time (D) in the RPP model ( n = 8). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, paired t test (A and B) or two-way ANOVA (C and D).

    Techniques Used:

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    Article Title: Chronic physical exercise increases a neurogenesis marker within hippocampus
    Article Snippet: .. Hippocampus BDNF level was determined by enzyme-linked immunosorbent assay (ELISA) method using Rat BDNF ELISA Kit from Elabscience. ..

    Article Title: A pharmacological rat model of recurrent pelvic pain exhibiting hyperalgesia and depression-like behaviors
    Article Snippet: Rat PGE 2 ELISA Kit , MEIMIAN , Cat# MM-0068R1. .. Rat BDNF ELISA Kit , Elabscience , Cat# E-EL-R1235. .. Rat IL-6 ELISA Kit , Elabscience , Cat# E-EL-R0015.

    Article Title: Extracts of Sideritis scardica and Clinopodium vulgare Alleviate Cognitive Impairments in Scopolamine-Induced Rat Dementia
    Article Snippet: .. The following kits were used: Rat BDNF ELISA Kit (cat. no. E-EL-R1235); Rat Phospho cAMP response element binding protein (P-CREB) ELISA Kit (cat. no. SL1344Ra), purchased from Elabscience (Houston, TX, USA). ..

    Article Title: Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain
    Article Snippet: .. ELISA measurement The levels of TNFα, (Rat TNF-α ELISA kit, Catalog No: E-EL-R0019, Elabscience Biotechnology Co. Ltd., USA), IL-4 (Rat IL-4 ELISA kit, Catalog No: E-EL-R0014, Elabscience Biotechnology Co. Ltd., USA), IL-6 (Rat IL-6 ELISA kit, Catalog No: E-EL-R0015, Elabscience Biotechnology Co. Ltd., USA), IL-10 (Rat IL-10 ELISA kit, Catalog No: E-EL-R0016, Elabscience Biotechnology Co. Ltd., USA), IL-17 (Rat Il-17 ELISA kit, Catalog No: E-EL-R0566, Elabscience Biotechnology Co. Ltd., USA), BDNF (Rat BDNF ELISA kit, Catalog No: E-EL-R1235, Elabscience Biotechnology Co. Ltd., USA), COX-2 (Rat COX-2 ELISA kit, Catalog No: E-EL-R0792, Elabscience Biotechnology Co. Ltd., USA), MMP-3 (Rat MMP-3 ELISA kit, Catalog No: E-EL-R0619, Elabscience Biotechnology Co. Ltd., USA) and TIMP-3 (Rat TIMP-3 ELISA kit, Catalog No: E-EL-R0986, Elabscience Biotechnology Co. Ltd., USA) in homogenised brain tissue samples (0.5 g) were determined using commercial ELISA kits and an ELISA reader (MWGt Lambda Scan 200, USA) following manufacturer’s instructions. ..

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    Article Snippet: .. The levels of BDNF were determined using Rat BDNF Elisa Kit (Elabscience Biotechnology Co, Ltd, Guangdong Science, and Technology, China) based on manufacturer instructions. ..

    Article Title: Neuroprotective Effects of Filgrastim in Rotenone-Induced Parkinson's Disease in Rats: Insights into its Anti-Inflammatory, Neurotrophic, and Antiapoptotic Effects.
    Article Snippet: All current treatments of Parkinson’s disease (PD) focus on enhancing the dopaminergic effects and providing symptomatic relief; however, they cannot delay the disease progression.. Filgrastim, a recombinant methionyl granulocyte colony-stimulating factor, demonstrated neuroprotection in many neurodegenerative and neurological diseases.. This study aimed to assess the neuroprotective effects of filgrastim in rotenone-induced rat model of PD and investigate the potential underlying mechanisms of filgrastim actions.

    Article Title: Neuroprotective Effect of Marrubium vulgare Extract in Scopolamine-Induced Cognitive Impairment in Rats: Behavioral and Biochemical Approaches
    Article Snippet: .. According to the manufacturer instructions, the following kits were utilized: the Rat BDNF ELISA Kit (cat. no. E-EL-R1235) from Elabscience and the Rat Phospho cAMP response element binding protein (pCREB) ELISA Kit (cat. no. SL1344Ra) from Sunlong Biotech Co., Ltd. (Hangzhou, China) Measurements were performed with a microplate reader set to 450 nm. ..

    Article Title: Expression of brain-derived neurotrophic factor in rapid ejaculator rats: A further study.
    Article Snippet: .. BDNF levels were determined by enzyme- linked immunosorbent assay (ELISA) method using Rat BDNF ELISA Kit (Elabscience, nloaded from https://onlinelibrary.w iley.com /doi/10.1111/and.14134 by < Shibboleth> -m em ber@ bcu.ac.uk, W iley O nline L ibrary on [01/05/2024]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Wuhan, Hubei, China) according to the protocol.

    Binding Assay:

    Article Title: Extracts of Sideritis scardica and Clinopodium vulgare Alleviate Cognitive Impairments in Scopolamine-Induced Rat Dementia
    Article Snippet: .. The following kits were used: Rat BDNF ELISA Kit (cat. no. E-EL-R1235); Rat Phospho cAMP response element binding protein (P-CREB) ELISA Kit (cat. no. SL1344Ra), purchased from Elabscience (Houston, TX, USA). ..

    Article Title: Neuroprotective Effect of Marrubium vulgare Extract in Scopolamine-Induced Cognitive Impairment in Rats: Behavioral and Biochemical Approaches
    Article Snippet: .. According to the manufacturer instructions, the following kits were utilized: the Rat BDNF ELISA Kit (cat. no. E-EL-R1235) from Elabscience and the Rat Phospho cAMP response element binding protein (pCREB) ELISA Kit (cat. no. SL1344Ra) from Sunlong Biotech Co., Ltd. (Hangzhou, China) Measurements were performed with a microplate reader set to 450 nm. ..



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    Persistent hyperalgesia in the RPP model (A and B) Paw withdrawal latency (A) and 50% paw withdrawal threshold (B) of each rat ( n = 3–6). (C) Representative histological images of DRG from T10-S4 by H&E staining. Arrow, Schwann cell; ∗, nuclei; #, cytoplasm. Scale bars, 50 μm and 25 μm. (D and E) Representative IHC images (D) and quantification (E) of <t>BDNF</t> in the DRG from T10-S4. Brown DAB staining indicates BDNF immunopositivity. The staining intensity was semi-quantitatively scored as follows: (1) weak, (2) moderate, and (3) intense. Scale bars, 50 μm. ( n = 3 biological replicates per group). (F) Serum BDNF level on days 12 and 24 of RPP modeling ( n = 3). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, two-way ANOVA (A and B) or one-way ANOVA (E and F).
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    Persistent hyperalgesia in the RPP model (A and B) Paw withdrawal latency (A) and 50% paw withdrawal threshold (B) of each rat ( n = 3–6). (C) Representative histological images of DRG from T10-S4 by H&E staining. Arrow, Schwann cell; ∗, nuclei; #, cytoplasm. Scale bars, 50 μm and 25 μm. (D and E) Representative IHC images (D) and quantification (E) of <t>BDNF</t> in the DRG from T10-S4. Brown DAB staining indicates BDNF immunopositivity. The staining intensity was semi-quantitatively scored as follows: (1) weak, (2) moderate, and (3) intense. Scale bars, 50 μm. ( n = 3 biological replicates per group). (F) Serum BDNF level on days 12 and 24 of RPP modeling ( n = 3). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, two-way ANOVA (A and B) or one-way ANOVA (E and F).
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    Persistent hyperalgesia in the RPP model (A and B) Paw withdrawal latency (A) and 50% paw withdrawal threshold (B) of each rat ( n = 3–6). (C) Representative histological images of DRG from T10-S4 by H&E staining. Arrow, Schwann cell; ∗, nuclei; #, cytoplasm. Scale bars, 50 μm and 25 μm. (D and E) Representative IHC images (D) and quantification (E) of <t>BDNF</t> in the DRG from T10-S4. Brown DAB staining indicates BDNF immunopositivity. The staining intensity was semi-quantitatively scored as follows: (1) weak, (2) moderate, and (3) intense. Scale bars, 50 μm. ( n = 3 biological replicates per group). (F) Serum BDNF level on days 12 and 24 of RPP modeling ( n = 3). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, two-way ANOVA (A and B) or one-way ANOVA (E and F).
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    Image Search Results


    Experimental Timeline: Rats were treated with EE, RJ, and EE + RJ for 14 consecutive days. Stress was induced on days 8–14. Behavioral tests were conducted on days 15–18. On day 19, the animals were decapitated to assess serum corticosterone and hippocampal BDNF levels.

    Journal: IBRO Neuroscience Reports

    Article Title: Pre-treatment with royal jelly, environmental enrichment, and their combination role in stress induced neurobehavioral deficits in male rats

    doi: 10.1016/j.ibneur.2026.03.012

    Figure Lengend Snippet: Experimental Timeline: Rats were treated with EE, RJ, and EE + RJ for 14 consecutive days. Stress was induced on days 8–14. Behavioral tests were conducted on days 15–18. On day 19, the animals were decapitated to assess serum corticosterone and hippocampal BDNF levels.

    Article Snippet: The evaluation of serum corticosterone levels and hippocampal BDNF levels was conducted using an enzyme-linked immunosorbent assay (ELISA) with a commercially available ELISA kit (E20160505043, Hangzhou Eastbiopharm Co. Ltd., Zhejiang Province, China) and rat BDNF ELISA kits from Boster Biological Technology Co. with a sensitivity limit of 4 pg/ml following the instructions provided by the manufacturer.

    Techniques:

    The effects of EE and RJ treatment on the hippocampal BDNF expression. The BDNF levels in the stressed group were significantly decreased than in the control group. EE and RJ treatment increased BDNF levels in the stressed rats. *, **, and *** represent p < 0.05, p < 0.01, and p < 0.001 v.s. the Non-stressed control group, respectively; ^^ and ^^^ represent p < 0.01 and p < 0.001 v.s. the Non-treatment stressed group, respectively.

    Journal: IBRO Neuroscience Reports

    Article Title: Pre-treatment with royal jelly, environmental enrichment, and their combination role in stress induced neurobehavioral deficits in male rats

    doi: 10.1016/j.ibneur.2026.03.012

    Figure Lengend Snippet: The effects of EE and RJ treatment on the hippocampal BDNF expression. The BDNF levels in the stressed group were significantly decreased than in the control group. EE and RJ treatment increased BDNF levels in the stressed rats. *, **, and *** represent p < 0.05, p < 0.01, and p < 0.001 v.s. the Non-stressed control group, respectively; ^^ and ^^^ represent p < 0.01 and p < 0.001 v.s. the Non-treatment stressed group, respectively.

    Article Snippet: The evaluation of serum corticosterone levels and hippocampal BDNF levels was conducted using an enzyme-linked immunosorbent assay (ELISA) with a commercially available ELISA kit (E20160505043, Hangzhou Eastbiopharm Co. Ltd., Zhejiang Province, China) and rat BDNF ELISA kits from Boster Biological Technology Co. with a sensitivity limit of 4 pg/ml following the instructions provided by the manufacturer.

    Techniques: Expressing, Control

    Persistent hyperalgesia in the RPP model (A and B) Paw withdrawal latency (A) and 50% paw withdrawal threshold (B) of each rat ( n = 3–6). (C) Representative histological images of DRG from T10-S4 by H&E staining. Arrow, Schwann cell; ∗, nuclei; #, cytoplasm. Scale bars, 50 μm and 25 μm. (D and E) Representative IHC images (D) and quantification (E) of BDNF in the DRG from T10-S4. Brown DAB staining indicates BDNF immunopositivity. The staining intensity was semi-quantitatively scored as follows: (1) weak, (2) moderate, and (3) intense. Scale bars, 50 μm. ( n = 3 biological replicates per group). (F) Serum BDNF level on days 12 and 24 of RPP modeling ( n = 3). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, two-way ANOVA (A and B) or one-way ANOVA (E and F).

    Journal: iScience

    Article Title: A pharmacological rat model of recurrent pelvic pain exhibiting hyperalgesia and depression-like behaviors

    doi: 10.1016/j.isci.2026.115059

    Figure Lengend Snippet: Persistent hyperalgesia in the RPP model (A and B) Paw withdrawal latency (A) and 50% paw withdrawal threshold (B) of each rat ( n = 3–6). (C) Representative histological images of DRG from T10-S4 by H&E staining. Arrow, Schwann cell; ∗, nuclei; #, cytoplasm. Scale bars, 50 μm and 25 μm. (D and E) Representative IHC images (D) and quantification (E) of BDNF in the DRG from T10-S4. Brown DAB staining indicates BDNF immunopositivity. The staining intensity was semi-quantitatively scored as follows: (1) weak, (2) moderate, and (3) intense. Scale bars, 50 μm. ( n = 3 biological replicates per group). (F) Serum BDNF level on days 12 and 24 of RPP modeling ( n = 3). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, two-way ANOVA (A and B) or one-way ANOVA (E and F).

    Article Snippet: Rat BDNF ELISA Kit , Elabscience , Cat# E-EL-R1235.

    Techniques: Staining

    Comorbid depression-like behavior in the RPP model (A and B) Serum BDNF (A) and 5-HT (B) levels in the RPP model ( n = 6). (C and D) Sucrose preference ratio (C) and immobility time (D) in the RPP model ( n = 8). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, paired t test (A and B) or two-way ANOVA (C and D).

    Journal: iScience

    Article Title: A pharmacological rat model of recurrent pelvic pain exhibiting hyperalgesia and depression-like behaviors

    doi: 10.1016/j.isci.2026.115059

    Figure Lengend Snippet: Comorbid depression-like behavior in the RPP model (A and B) Serum BDNF (A) and 5-HT (B) levels in the RPP model ( n = 6). (C and D) Sucrose preference ratio (C) and immobility time (D) in the RPP model ( n = 8). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, paired t test (A and B) or two-way ANOVA (C and D).

    Article Snippet: Rat BDNF ELISA Kit , Elabscience , Cat# E-EL-R1235.

    Techniques: